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  技术文章 河北快3 >> 技术文章
冰冻切片线粒体内膜功能操作流程
点击次数:103 发布时间:2019-11-04

HL10013.6 v.A

 

河北快3BINGDONGQIEPIANXIANLITINEIMOGONGNENG(MODIANWEI)YINGGUANGCEDINGSHIJIHECHANPINSHUOMINGSHU

 

ZHUYAOYONGTU

 

河北快3BINGDONGQIEPIANXIANLITINEIMOGONGNENG(MODIANWEI)YINGGUANGCEDINGSHIJISHIYIZHONGZHIZAITONGGUOGAODUMINGANDEYANGLIZIYINGGUANGTANGHUAQINGRANLIAOJIEHEDAOXIANLITIJIZHI,QIYINGGUANGDEZENGQIANGHUOJIANRUOSHUOMINGXIANLITIMODIANWEIDEBIANHUA,JINEIMODIANFUXINGDEZENGGAOHUOJIANGDI,LAIFENXIBINGDONGZUZHIYANGPINXIANLITINEIMOGONGNENGDEWANZHENGXINGDEERJINGDIANDEJISHUFANGFA。GAIJISHUJINGGUOJINGXINYANZHI、CHENGGONGSHIYANZHENGMINGDE。QISHIYONGYUGEZHONGBINGDONGDONGWUZUZHIXIANLITIDEGONGNENGJIANCE。KEYIBEIYONGYUXIBAODIAOWANG、XINHAOCHUANDI、SHUAILAODENGDEYANJIU。CHANPINYANGEWUJUN,JIDAOJIYONG,HUOTIJIANCE,CAOZUOJIWEIJIANYI,XINGNENGWENDING。

 

JISHUBEIJING

 

河北快3YANGLIZIYINGGUANGTANGHUAQINGRANSEJIJC-1(5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide)SHIYIZHONGDUIMODIANWEIGAODUMINGANDERANSEJI。TATEYIXINGDIJINRUXIANLITI,FENBUHEJIEHEZAIXIANLITIJIZHISHANG。XIANLITIMODIANWEIDEGAODIBIANHUAJUEDINGLEJC-1DEFENBUNONGDU。DIANWEIGAO,JC-1XINGCHENGJUJITI,ERNONGDUGAO,YINGGUANGXIANSEWEIHONGSEHUOJIEHONGSE;FANZHI,ZEWEILVSE。YINGGUANGJIANRUOBIAOMINGXIANLITINEIMOGONGNENGSHOUDAOSUNHAI。 

 

CHANPINNEIRONG

 

河北快3RANSEYE(Reagent A)     40WEISHENG

XISHIYE(Reagent B)      5HAOSHENG

QINGLIYE(Reagent C)      20HAOSHENG

CHANPINSHUOMINGSHU       1FEN

 

BAOCUNFANGSHI

 

河北快3BAOCUNQINGLIYE(Reagent C)ZAI4℃BINGXIANGLI,QIYUDEBAOCUNZAI-20℃BINGXIANGLI;RANSEYE(Reagent A)BIMIANGUANGZHAO;YOUXIAOBAOZHENG6YUE

 

YONGHUZIBEI

 

1.5HAOSHENGLIXINGUAN:YONGYUGONGZUOYEPEIZHIDERONGQI

PEIYANGXIANG:YONGYURANSEFUYU

(GONGJUJIAO)YINGGUANGXIANWEIJING:YONGYUBINGDONGQIEPIANZUZHIXIBAOXIANLITIYINGGUANGDINGXINGFENXI

 

SHIYANBUZHOU

 

SHIYANKAISHIQIAN,JIANG-20℃BINGXIANGLIDESHIJIHEZHONGDERANSEYE(Reagent A)ZHIRUBINGCAOLIRONGHUA,XISHIYE(Reagent B)FANGJIN37℃HENGWENSHUICAOLIYURE。RANHOUYICHU10WEISHENGRANSEYE(Reagent A)DAOXINDE1.5HAOSHENGLIXINGUAN,JIARU890WEISHENGXISHIYE(Reagent B),HUNYUNHOU,ZAIBINGCAOLIJINGZHI,BINGBIAOJIWEIRANSEGONGZUOYE,FANGZAIANSHILI。RANHOUJINXINGXIALIECAOZUO。

 

河北快31.ZHUNBEI5PIANDAICEDEHOUWEI10WEIMIDEWEIJINGGUZHECHULIDEBINGDONGQIEPIAN

河北快32.ZHIYUSHIWENXIA,XIAOXINJIASHANG500WEISHENGYULENGDEQINGLIYE( Reagent C),PUMANZHENGGEQIEPIANBIAOMIAN

3.XIAOXINYIQUQIEPIANSHANGDEQINGLIYE(Reagent C)

4.XIAOXINJIASHANG200WEISHENGSHIWENYUREDERANSEGONGZUOYE,PUMANZHENGGEQIEPIANBIAOMIAN

5.ZAI37℃SHIRUNPEIYANGXIANGLI,FUYU20FENZHONG(ZHUYI:BIMIANYETIZHENGFA)

6.XIAOXINYIQUQIEPIANSHANGDERANSEGONGZUOYE,BIMIANGUANGZHAO

7.XIAOXINJIASHANG500WEISHENGQINGLIYE(Reagent C),PUMANZHENGGEQIEPIANBIAOMIAN

8.XIAOXINYIQUQIEPIANSHANGDEQINGLIYE(Reagent C)

9.FANGSHANGGAIBOPIANHUOFENGPIAN(ZHUYI:JIANCEQIANZHIYUBINGCAOLI)

河北快310.JIKEZAIDAOZHIYINGGUANGXIANWEIJINGXIAJINXINGGUANCHA(DANLVBOHUOSHUANGLVBO):

 

GUANCHAHONGSEYINGGUANG:LVBOQIJIFABOZHANG490nm,SANFABOZHANG590nmHUOLUODANMING(rhodamine)LVBOQIJIFABOZHANG540nm,

河北快3              SANFABOZHANG570nmHUODEZHOUHONG(Texas Red)LVBOQIJIFABOZHANG590nm,SANFABOZHANG610nm--KEJIAN

              LIANGHONGSEYINGGUANG;RUGUOQIANGDUXIANZHUJIANRUO,BIAOMINGXIANLITIMODIANWEISHOUDAOPOHUAI

 

河北快3GUANCHALVSEYINGGUANG:LVBOQIJIFABOZHANG490nm,SANFABOZHANG530nmHUOYINGGUANGSU(Fluorescein)LVBOQIJIFABOZHANG490nm,

河北快3              SANFABOZHANG520nm--RUGUOLVSEYINGGUANGQIANGDUXIANZHUZENGQIANG,BIAOMINGXIANLITIMODIANWEISHOUDAOPOHUAI,WEIJIEHEJC

河北快3              -1RANLIAOZAIXIBAOJIANGLI

ZHUYISHIXIANG

 

1.BENCHANPINWEI20CICAOZUO

2.CAOZUOSHI,XUDAISHOUTAO

河北快33.RANSEGONGZUOYEBIMIANGUANGZHAO

4.YONGHUGENJUSHIJIXUQIU,ANBILIPEIZHISHIJIYONGLIANG

5.GENJUBUTONGZUZHILEIXING,DIAOZHENGRANSEGONGZUOYEDESHIYONGJILIANG(1:100ZHI1:1000RONGLIANGBI),BIMIANGUODURANSE

6.JIANYIQIEPIANZUZHIRANSEWANCHENGHOU,JIKEJINXINGYINGGUANGJIANCEFENXI

7.FUYUSHI,BIMIANGUANGZHAO

河北快38.SHUANGLVBOHUOSHUANGZHONGCEDING:JIANKANGXIBAOHEXIANLITICHENGXIANHONGSE;SIWANGHUODIAOWANGXIBAOJISUNSHANGXIANLITICHENGXIANLVSE

9.BENGONGSITIGONGXILIEXIANLITISHIJICHANPIN

 

ZHILIANGBIAOZHUN

 

河北快31.BENCHANPINJINGJIANDINGXINGNENGWENDING

河北快32.BENCHANPINJINGJIANDINGYINGGUANGQINGXI

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